Table 2.
Cell molecules for circulating microvesicle (cMV) identification and characterization.
| mAb | Alternative Name | Expression | Conjugation | Clone | Company |
|---|---|---|---|---|---|
| AV | PS-binding protein | Widely expressed | APC | BD Biosciences | |
| CD142 | Tissue Factor | Widely expressed | FITC | VD8 | Sekisui diagnostics |
| Platelet-Related | |||||
| CD61 | β3-integrin | Platelets | PE | VI-PL2 | BD Pharmingen |
| CD31 | PECAM-1 | Activated Cells | FITC | AC128 | Miltenyi Biotec |
| CD62P | P-Selectin | Platelets | PE | HI62P | Immunotools |
| CD42b | Glycoprotein Ib alpha chain | Platelets and megakaryocytes | PE | REA185 | MACS Miltenyi |
| Endothelial Cell-Related | |||||
| CD146 | Melanoma Cell Adhesion Molecule | Endothelial Cells | FITC | P1H12 | BD Pharmingen |
| CD62E | E-Selectin | Endothelial Cells | PE | 68-5H11 | BD Biosciences |
| CD309 | VEGFR-2 | HSCs, EPCs, and ECs | FITC | ES8-20E6 | MACS Miltenyi |
| Erythrocyte-Related | |||||
| CD235ab | Glycophorin A and B | Erythrocytes | FITC | HIR2 | Immunotools |
| Leukocyte-Related | |||||
| CD45 | Leukocyte Common Antigen | Leukocytes | PE | MEM-28 | Immunotools |
| CD3 | T-cell co-receptor | T-Lymphocytes | FITC | HIT3b | Immunotools |
| CD14 | LPS-receptor | Macrophages, monocytes | PE | M5E2 | BD Pharmingen |
| CD62L | L-Selectin | Leukocytes | FITC | LT-TD180 | Immunotools |
| CD11b | Macrophage-1 Antigen (Mac-1) | Neutrophils, leukocytes | FITC | M1/70.15.11.5 | MACS Miltenyi |
| Smooth Muscle Cell-Related | |||||
| SMA-α | Smooth Muscle Actin α | Smooth muscle cells | PE | 1A4 | R&D Systems |
APC denotes allophycocyanin; AV, Annexin V; CD, cluster of differentiation; ECs, endothelial cells; EPCs, endothelial progenitor cells; FITC, fluorescein isothiocyanate; HSCs, hematopoietic stem cells; LPS, lipopolysaccharide; mAb indicates monoclonal antibody; PE, phycoerythrin; PECAM-1, platelet endothelial cell adhesion molecule; PS, phosphatidylserine; and VEGFR-2, vascular endothelial growth factor receptor-2.