Figure 4.

Schematic representation of an approach of genome editing (GE) with Cas9/sgRNA. First, the target gene is selected; sgRNAs are designed and synthesized using online tools. Generally, cloning of target sgRNA with Cas9 (or with its variant) is performed using a plant binary vector for Agrobacterium-mediated transformation into target plant species. Transformed plants are then selected for the presence of Cas9 and sgRNA, followed by PCR/RE genotyping. Finally, DNA sequencing is used for selecting the plants with the desired editing/mutation.