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. 2019 May 21;20(10):2500. doi: 10.3390/ijms20102500

Figure 2.

Figure 2

Time-dependent release of CK2 from BMPRIA at 12 h and 18 h post CK2.3 stimulation. (A) Visual analysis of the effect of CK2.3 on the interaction between endogenous BMPRIA and CK2α. Confocal images of fixed C2C12 cells that were either (A) unstimulated (-) or stimulated with 100 nM of CK2.3 (+) for (B) 6 h, (C) 12 h, and (D) 18 h. The images depict the interaction between endogenous BMPRIA (green) and CK2α (red) within the cell at different time intervals. The nucleus of the cells is depicted in blue. (E) In the secondary control, unstimulated cells were stained using only the fluorescent secondary antibody to determine their specificity, and lack of staining in the cells shows that the antibody was specific against the antigen. (B) Immuno-precipitation of BMPRIA in C2C12 cells, not stimulated (-) or stimulated (+) with 100 nM of CK2.3 for 6 h, 12 h, and 18 h; followed by a co-immuno-precipitation for CK2β that showed reduced interaction of CK2β with BMPRIA at 12 h and 18 h post-stimulation. Con represents the negative control of the immuno-precipitation, where lysis buffer was used instead of cell lysate.