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. 2019 May 21;20(10):2500. doi: 10.3390/ijms20102500

Figure 6.

Figure 6

200 pM of Smad4 siRNA induces significant reduction in mineralization without affecting the viability of C2C12 cells. (A) Mineralization levels in unstimulated C2C12 cells and cells treated with 200 pM of control siRNA and 200 pM of Smad4 siRNA, followed by stimulation with 100 nM of CK2.3, was determined using von Kossa assay. (B) Viability of cells and (C) total number of cells after treatment with 200 pM of control siRNA and 200 pM of Smad4 siRNA followed by stimulation with 100 nM of CK2.3 was determined using MTT assay and imageJ software, respectively. Data (n = 3) was normalized to unstimulated cells and analyzed using one-way anova and Tukey-Kramer post hoc statistical test (p < 0.05). (A) and (C) a = statistically significant difference to unstimulated, b = statistically significant difference to 100 nM CK2.3, c = statistically significant difference to 200 pM control siRNA + 100 nM CK2.3, and d = statistically significant difference to 200 pM Smad4 siRNA + 100 nM CK2.3. (B) a = statistically significant difference to control, b= statistically significant difference to unstimulated, c = statistically significant difference to 100 nM CK2.3, d = statistically significant difference to 200 pM control siRNA + 100 nM CK2.3, and e = statistically significant difference to 200 pM Smad4 siRNA + 100 nM CK2.3.