Effects of ciwujianoside C3, eleutheroside M and ciwujianoside B on object recognition memory in mice. (A) Ciwujianoside C3 (C3), eleutheroside M (M), ciwujianoside B (B) (each 0.5 mg/kg, p.o.) or vehicle solution (Veh) was administered for 17 days to mice (males, 8 weeks old). On administration day 14, an object recognition test was started. (B) The preference indices of the training and test sessions are shown. (* P < 0.05, ** P < 0.01, *** P < 0.001, repeated measures two-way ANOVA, post hoc Bonferroni test; n = 5 mice). Above 50% of the preferential index in a test session shows memory enhancement. (C) Locomotion was evaluated as moved distances for 10 min in an open field box on day14. Statistical analysis was done by one-way ANOVA. P > 0.05. (D) Body weights in four groups were measured and analyzed by repeated measures two-way ANOVA. P > 0.05.