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. 2019 May 11;11(5):1061. doi: 10.3390/nu11051061

Figure 2.

Figure 2

Suppression of XIAP by SC F2 sensitizes cells to TRAIL-mediated apoptosis. (A) DLD-1 cells were treated with the indicated concentrations of SC F2 for 20 h. Western blotting of death receptor (DR4, DR5), anti-apoptotic and pro-apoptotic protein. (B) DLD-1 cells were treated with indicated SC F2 doses for 20 h. Cell lysates were analyzed by Western blotting using anti-caspase 9, anti-caspase 3, and anti-cleaved PARP-1 antibodies. (C) DLD-1 and HCT116 cells were exposed to 200 µg/mL of SC F2 for the indicated time intervals. Cell lysates were analyzed by Western blotting using an anti-XIAP antibody. (D) XIAP was silenced by XIAP siRNA in DLD-1 cells. The cells were then treated with TRAIL for 4 h followed by flow cytometry analysis. Error bars represent standard error of the mean (SEM) from three separate experiments. “*” represents a statistically significant difference between TRAIL + siCon-treated and TRAIL + siXIAP-treated cells at p < 0.05. (E) XIAP was silenced by XIAP siRNA in DLD-1 cells. The cells were then treated with TRAIL for 4 h followed by Western blotting. ** p < 0.005.