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(A) Combined yield of Cys-MeCA isomers (3-S-Cys-5-MeCA, 4-S-Cys-5-MeCA and diCys-MeCA) after HCl hydrolysis of MeCA conjugates with BSA or βLG after 3 min and 60 min. (B) Combined yield of Cys-DA isomers (5-S-Cys-DA, 2-S-Cys-DA, 6-S-Cys-DA and 2,5-S,S’-diCys-DA) after HCl hydrolysis of DA conjugates with BSA or βLG after 3 min and 60 min. Data are means (n = 4) and standard error of the mean (SEM) from 2 experiments, each hydrolysate being analyzed in duplicate.
