Table 2.

AOA treatment inhibits H₂S production in primary spinal cord cultures.

H2S (MG/L)	Days in Culture
	II	IV	V	VI	VII
NT (Not Treated)	4.3 ± 0.4	4.5 ± 0.03	3.8 ± 0.26	3.2 ± 0.14	1.42 ± 0.16
250 µM AOA	2.7 ± 0.2	2 ± 0.02	1.4 ± 0.015	0.7 ± 0.21 *	ND
500 µM AOA	1.8 ± 0.25 *	0.9 ± 0.08 *	ND	ND	ND
1 MM AOA	0.4 ± 0.26 *	ND	ND	ND	ND
2 MM AOA	ND	ND	ND	ND	ND

Effects of the CSE/CBS inhibitor AOA on the production of H₂S in primary spinal cord cultures. The levels of H₂S values (mg/L) in the culture media were measured as described in Material and Methods and are presented as medians ± SE. ND = not detectable; *p < 0.05 versus the values in the NT group of the corresponding day.