Fig. 1.

Developmental and experimental schematic. a Example phase images show organoids at early stages of development, becoming more structured, and organoid appearance as balls of tissue in an Erlenmeyer culture flask. H&E staining at 28 days old (do) shows the complexity of the organoids with many varied, structured domains. Immunofluorescence at 140 do confirms cortical identity (FoxG1) and shows that astrocytes (GFAP) have begun populating the neuronal layers. Scale bars = 200 μm. b Schematic time line of organoid maturation, infection and sampling