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. 2019 Jun 14;7:12. doi: 10.1186/s40478-019-0742-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunohistochemical staining for PrP in organoids harvested at 169 dpi. a Whole organoid images; left panels per condition show 6H4 PrP staining (brown) and right panels showing positive pixel counts (PPC), blue signifies low and yellow-orange denotes high pixel staining intensity. Scale bars = 500 μm. b PrP positive pixel counts. Each data point represents an individual organoid with the mean indicated as a line (Kruskal-Wallis, p = 0.132). c 6H4 PrP staining of organoid interiors. Scale bar = 50 μm and applies to left panels. Right panels are zoomed images of the boxed regions. d PrP staining artifacts visible in NBH and MV2 treated organoids. Left panels show 6H4 PrP staining and right panels show no-primary antibody control stains. Arrows indicate artifacts in sequential slices. Scale is as for C