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. 2019 Jun 13;38:256. doi: 10.1186/s13046-019-1260-6

Fig. 5.

Fig. 5

MiR-20a reverses the tumor suppressive function of PTENP1 by regulating PTEN expression in BC progression. a Relative PTEN expression was measured by qRT-PCR in the cells transfected with miR-20a mimic or PTENP1. b PTEN protein level was detected by western blot. c The variety of growth rates were confirmed by CCK8 assay. d Colony formation assay was conducted to show the proliferative formation with different treated BC cell lines. e The altered migration and invasion were detected by transwell assay (Scale bar = 20 μm). f The chemoresistance to ADR was identified by CCK8 assay. g The IC50 values of different groups were calculated. h The colony formation was shown in MCF-7/ADR cells in response to ADR. i The altered apoptotic rate was detected by flow cytometry. Data are means ± SD of three independent assays (*P < 0.05)