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. 2019 Jun 13;18:109. doi: 10.1186/s12934-019-1156-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The set of four reactions, panels a–d, belonging to Category 4 (C4). C4 reactions and derivatives are neither present in iML1515 nor associated with any other organism in KEGG or EcoCyc. Each of the four panels is divided into three sections (I) the balanced reaction developed by our workflow indicating the reactants, products, and the promiscuous enzyme, (II) the RDM pattern showing the Reaction Center (R) in red, and (III) the native reaction catalyzed by the potentially promiscuous enzyme, as catalogued in KEGG