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. 2019 Jun 13;10:171. doi: 10.1186/s13287-019-1293-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — DFX enhances BFU-E colony formation proficiency of CD34⁺ HS/PCs. Quantitative analysis of CFU-GEMM, CFU-GM and BFU-E derived by CD34⁺ cells isolated from human umbilical cord blood (hUCB; upper panel) or mobilized bone marrow-derived from adult healthy subjects (hmBM; lower panel) and cultured without DFX, with 20 μM DFX and with 20 μM DFX + NAC 10 mM. DFX and NAC were added to methylcellulose-based medium at time of plating, and colonies were detected and enumerated after 14 days of culture. Results shown represent the mean ± SD of three independent experiments. (*p < 0.05 vs CTRL). Representative images of CFU-GEMM, CFU-GM and BFU-E scored under an inverted microscope (magnification × 40) are shown