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. 2019 Jun 13;10:171. doi: 10.1186/s13287-019-1293-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — DFX affects colony formation ability and promotes expression of differentiation markers in leukemia cell lines. a Colony formation assay in Kasumi-1, K562 and HL60 treated with DFX and DFX + NAC. Representative images of colonies scored under an inverted microscope (magnification × 40) are shown on the top; left photo: CFU-GM from Kasumi-1 cells; central photo: BFU-E from K562 cells; right photo: CFU-GM from HL-60 cells. On the bottom, quantitative analysis of colonies derived by each cell line cultured without DFX, with 20 μM DFX and with 20 μM DFX + NAC 10 mM for 14 days in a methylcellulose-based medium. Colony number is shown as the mean ± SD of three independent experiments (*p < 0.05 vs CTRL). b Representative flow cytometric analysis of CD36 (on the left) and CD14 (on the right) distribution in untreated (CTRL—black area), DFX-treated (white area) and DFX + NAC (grey area) Kasumi-1 cells for 48 h. c Representative flow cytometric analysis of CD36 (on the left) and CD71 (on the right) distribution in untreated (CTRL—black area), DFX-treated (white area) and DFX + NAC (grey area) in K562 cells 48 h. d Representative flow cytometric analysis of CD11b (on the left) and CD16 (on the right) distribution in untreated (CTRL—black area), DFX-treated (white area) and DFX + NAC (grey area) in HL60 cells for 48 h. Per marker, histograms concerning both percentage of positive cells and MFI (expressed as mean ± SEM) are shown (*p < 0.05 vs CTRL, ^#p < 0.05 vs DFX)