Table 1.

Culture formula optimization for human CD34⁺ cell expansion

Group	IMDM	Nutrition Supplements	SCF (ng/mL)	Flt-3 L (ng/mL)	TPO (ng/mL)	IL-3 (ng/mL)	G-CSF (ng/mL)	GM-CSF (ng/mL)	SR1 (μM)
GF0	+	–	–	–	–	–	–	–	–
SGF0	+	+	–	–	–	–	–	–	–
SGF2	+	+	200	200	0	–	–	–	–
SGF3	+	+	200	200	5	–	–	–	–
	+	+			10	–	–	–	–
	+	+			20	–	–	–	–
	+	+			50	–	–	–	–
	+	+			100	–	–	–	–
SGF4	+	+	200	200	20	5	–	–	–
	+	+				10	–	–	–
	+	+				15	–	–	–
	+	+				25	–	–	–
	+	+				50	–	–	–
	+	+				100	–	–	–
SGF5	+	+	200	200	20	15	5	–	–
	+	+					10	–	–
	+	+					15	–	–
	+	+					25	–	–
	+	+					50	–	–
	+	+					100	–	–
SGF6	+	+	200	200	20	15	10	5	–
	+	+						10	–
	+	+						15	–
	+	+						25	–
	+	+						50	–
	+	+						100	–
SGF7	+	+	200	200	20	15	10	10	0.5
	+	+							1
	+	+							2
HEM	+	+	200	200	20	15	10	10	–

The composition of the base (GF0 and SGF0), intermediate (SGF2-SGF7), and the final optimized media formula (HEM) are shown