Table 3.
Primers used in this study
| Primer name | Nucleotide Sequence | Primer location (nt) | Product length (bp) | Purpose | Resource |
|---|---|---|---|---|---|
| PCV3-D-F | CCACAGAAGGCGCTATGTC | 1909–1927 a | 330 | Detection | [4] |
| PCV3-D-R | CCGCATAAGGGTCGTCTTG | 1599–1617 a | |||
| PCV3-N2-F | AGGAGGTTCACTAAGGTTGT | 1026–1045 a | 1313 | Genome sequencing (nested PCR For G2 fragment) | This study |
| PCV3-N2-R | CTCTTTGCCGATAATAAGGTAT | 317–338 a | |||
| PCV3-G2-F | TTGCACTTGTGTACAATTATTGCG | 1113–1136 a | 1075 | [3] | |
| PCV3-G2-R | ATCTTCAGGACACTCGTAGCACCAC | 2163–0-187 a | |||
| PCV3-G1-F | TAGTATTACCCGGCACCTCGGAACC | 1994-0-18 a | 1257 | Genome sequencing (nested PCR For N1 fragment) | [3] |
| PCV3-G1-R | ACAGGTAAACGCCCTCGCATGTGGG | 1226–1250 a | |||
| PCV3-N1-F | GATGAAGCGGCCTCGTGT | 106–123 a | 1064 | This study | |
| PCV3-N1-R | CACCCACCCTCCCAATAA | 1152–1169 a | |||
| PCV3-Cap-F | ATGAGACACAGAGCTATATT | 1961–1980 a | 645 | Cap gene sequencing | This study |
| PCV3-Cap-R | TTAGAGAACGGACTTGTAAC | 1336–1355 a | |||
| PCV2-F | AAGGGCTGGGTTATGGTATG | 1621–1640 b | 353 | Detection | This study |
| PCV2-R | CGCTGGAGAAGGAAAAATGG | 187–206 b |