Fig. 4.

Transient expression of constitutive active STAT1 restores MHC-I expression on OGD stressed tumor cells. B16F10 tumor cells were transiently overexpressed with STAT1-WT or STAT1-cc using e-GFP as a control, prior to culturing the cells under normal or OGD culture conditions for 24 h with IFNy stimulation. a Western-blot results of endogenous STAT1 expression and exogenous STAT1 overexpression after transfection of STAT1-WT, STAT1-cc, or control. b Histogram illustrating MHC-I surface expression of B16F10 tumor cells cultured under normal or OGD conditions measured by flow cytometry. Representative data is shown as mean + − SD (n = 3). c Quantified data of B is shown as MFI. Unpaired t test was used to calculate significance; n.s., not significant *, p < 0.05, **, p < 0.01, ***, p < 0.001