Fig. 1.

Tumor infiltrating B cell density is not associated with response to anti-PD-1. a, b Tumor is masked by antibodies to S100 and HMB45 conjugated to Cy2 (green), and the area of B cell infiltrate is determined by the relative area of CD20 positive cells within the tumor mask, identified by anti-CD20 antibodies conjugated to Cy5 (red). Nuclei are identified by DAPI (blue). Examples of high B cell density (a) and low B cell density (b) are shown. c B cell quantification in responders (CR: complete response, PR: partial response) (n = 11) vs non-responders (SD: stable disease, PD: progressive disease) (n = 23), p = 0.22, by unpaired t-test. d Presence or absence of tumor infiltrating B cells does not correlate with overall survival, log-rank p = 0.64