Fig. 1.

β-TrCP associated with ARID1A. a HEK293T cells were transfected with empty vector (EV) or the indicated FLAG-tagged F-box protein plasmids (FBPs). Cell extracts were immunoprecipitated (IP) with anti-FLAG M2 beads, and the immunocomplexes were subjected to western blot with the indicated antibodies. b HEK293T cells were transfected with Flag-ARID1A for 36 h, cell extracts were IP with anti-FLAG M2 beads, and the immunocomplexes were subjected to western blot with the indicated antibodies. c HEK293T cells were co-transfected with Flag-ARID1A and HA-β-TrCP WT or HA-β-TrCP R474A mutant for 36 h, cell extracts were IP with anti-HA beads, and the immunocomplexes were subjected to western blot with the indicated antibodies