Figure 6.

Effect of IL-25 on the expression of fibroblast-derived profibrotic mediators. Serum-starved human lung fibroblasts were stimulated with various dosages of hIL-25 for indicated time points. TGF-β1 was used for the positive control. COL1A1 (a), COL3A1 (b), fibronectin (c), α-SMA (d), CTGF (e), TIMP-1 (f), MMP-1 (g), and MMP-9 (h) mRNA levels were evaluated by real-time RT-PCR. Data are presented as a fold change compared with vehicle controls at the same time point. Values are means ± SEMs from three individual experimental replicates (*P < .05, **P < .01, ***P < .001 compared with vehicle-treated cells by using ANOVA). α-SMA: α smooth muscle; CTGF: connective tissue growth factor; IL-25: interleukin 25; TGF-β1: human transforming growth factor-β1; TIMP: tissue inhibitor of metalloproteinase; MMP: matrix metalloproteinase. (A color version of this figure is available in the online journal.)