Fig. 3.

Expression of Various circRNAs in the testes treated with poly I:C. a Hierarchical clustering assessment of circRNAs that displayed variation in expression patterns between control and poly I:C treatment groups; every group comprised three individuals (over two-fold difference in expression; P < 0.05). Expression values are shown in various colors suggestive of high and low median expression levels. b Scatter plot was used to evaluate alterations in circRNA expression between control (group A) and poly I:C treatment (group B) specimens. Values corresponding to X and Y axes in the scatter plot were normalized signal values of specimens (log₂ scaled). Green lines indicate fold alterations. CircRNAs over the top green line and below the bottom green line indicate over two-fold changes. c Volcano plots were built to show fold change values and P values. The vertical lines show two-fold upregulation and downregulation between control and poly I:C treatment specimens (A versus B), and the horizontal line shows P value. The red point in the plot shows various expression patterns of circRNAs with statistical significance