Fig. 5.

Effect of circRNA-9119 inhibition on the transcription and translation of inflammation-associated genes in testicular cells. a Sertoli and Leydig cells were separated from C57BL/6 mice and treated with circRNA-9119 siRNA or NC siRNA prior to treatment with poly I:C or PBS for 6 h. Total RNA and proteins were isolated from cells, and circRNA-9119 level was evaluated with qRT-PCR after normalization to GAPDH level. b and c Western blotting was used to investigate the stimulation of P65 and IRF3 expression and to evaluate the expression of total P65 and IRF3. d and e qRT-PCR was used to detect the mRNA levels of cytokines after 6 h treatment with poly I:C. Results are expressed as means ± SEM (n = 3 replicates each experiment). *P < 0.05, **P < 0.01, ***P < 0.001