Fig. 7.

miR-26a and miR-136 contributed to circRNA-9119-mediated inflammatory reactions. a-d Sertoli and Leydig cells were separated from C57BL/6 mice and co-transfected with circRNA-9119 siRNA and/or miR-26a/miR-136/NC siRNA, prior to treatment with poly I:C or PBS for 6 h. Total RNA was isolated from cells and miR-26a and miR-136 levels were evaluated with qRT-PCR after normalization to GAPDH level. e and f qRT-PCR was used to evaluate the expression of IL-1β, MCP-1, and IFN-β in Sertoli and Leydig cells after 6 h of poly I:C treatment. Results are expressed as means ± SEM (n = 3 replicates each experiment). *P < 0.05, **P < 0.01, ***P < 0.001