Table 3.
Statistical evaluation AutoCryoPicker and EMAN2 performance using the Apoferritin and KLH images
| Cryo-EM images | Particle Shape | Total Particles Number | AutoCryoPicker | EMAN2 | ||||
|---|---|---|---|---|---|---|---|---|
| TP | FN | FP | TP | FN | FP | |||
| Apoferritin Image 1 | Top-View | 151 | 148 | 3 | 2 | 84 | 67 | 0 |
| Apoferritin Image 2 | Top-View | 160 | 159 | 1 | 5 | 83 | 76 | 1 |
| KLH image 1 | Top-View | 17 | 14 | 3 | 0 | 8 | 9 | 11 |
| KLH image 2 | Top-View | 7 | 7 | 0 | 0 | 3 | 4 | 10 |
| KLH image 1 | Side-View | 24 | 8 | 15 | 0 | N/A | N/A | N/A |
| KLH image 2 | Side-View | 14 | 6 | 8 | 0 | N/A | N/A | N/A |
The table reports TP: True Positive picking results where the correct particles are picked, FN: False Negative picking results where some good particles are missed, FP: False Positive picking results where the incorrect particles (other objects such as background or artificial objects) are picked as particles