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Resorption lacunae on dentin after staining with toluidine blue. Lack of resorption pits in monolayer PBMC/PPF co-cultures (A), even when cultivated in the presence of additional MCSF and RANK-L (B). PBMC monocultures stimulated with MCSF and RANK-L, cultivated in monolayer (C) or on transwell membranes (and transferred on dentin, (D)), showed extensive traces of osteolysis.
