Figure 7.

Immunohistochemical and immunofluorescence staining for CD31 and OCN in the defects implanted with the BG and 3Co-BG scaffolds and in the unfilled defects (control) at 8 weeks post-implantation. Blood vessels were defined by positive CD31 staining or red fluorescence and their typical round or oval structure. There were more new vessels (red arrows and red fluorescence) in the defects implanted with the 3Co-BG scaffolds (C, I) than for the BG group (B, H) or the control group (A, G). There were almost no positive staining or green fluorescence for OCN in the control group (D, J), a limited amount in the BG group (E, K), and a higher amount 3Co-BG group (F, L).