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. 2019 May 11;15(6):1252–1260. doi: 10.7150/ijbs.28235

Figure 5.

Figure 5

C3A-iCSCs acquired higher proliferation and migration. A-B. Flow cytometric analysis of the cell cycle of C3A cells and C3A-iCSCs. C. Nuclear EdU staining of C3A and C3A-iCSCs. Scale bar, 100 μm. Percentages of EdU-positive cells were calculated, n=5. D. Transwell assay performed in C3A and C3A-iCSCs. Cells were starved 24 hrs before seeding into tranwell chamber; images were captured 48 hrs later after seeding. Cells of lower layer of chamber were counted, n=5. E. Wound-healing assay performed in C3A and C3A-iCSCs. Quantities analysis of wound-healing assay were performed, n=5. F. Real-time PCR analysis of the EMT-related genes in C3A cells and C3A-iCSCs. (* p<0.05, *** p<0.001, ns indicated no significant differences)