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. 2019 May 11;15(6):1240–1251. doi: 10.7150/ijbs.33044

Figure 4.

Figure 4

Effect of GDM on the ROS level in offspring. Heart tissues were isolated from male offspring. (A) ROS levels in the left ventricle (LV) tissues isolated from both control (□) and STZ-treated (■) groups were measured using in vitro ROS/RNS assay kit. (B) NOX1, 2, and 4 protein abundances in the LV tissues isolated from both control (□) and STZ-treated (■) groups were determined by Western blot analysis. Their protein densities were normalized to internal control (GAPDH). (C) After NAC pretreatment, ROS levels in the LV tissues isolated from both control (□) and STZ-treated (■) groups were measured using in vitro ROS/RNS assay kit. Data are means ± SEM (n=4 animals/group). *P < 0.05 vs. control, as determined by Student's t-test.