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. 2019 May 7;15(6):1177–1186. doi: 10.7150/ijbs.33103

Figure 4.

Figure 4

Overexpression of PCBP1 reduces the ratio of STAT3α/β and suppresses the cell proliferation of a breast cancer cell line (MCF-7). (A, B) MCF-7 cells were stably transfected with T7 tagged PCBP1 or control lentivirus and seeded into 12 well plates on Day 0. Cell numbers were counted on Day 2 and Day 4 (A). (B) The histograms show significant difference between the PCBP1 overexpression and control groups on Day 4. Data are the means ± SE, n =3. (C) The alternative splicing of exon 23 was detected by RT-PCR. Relative α/β represents the ratio of band intensities of α vs β isoform. GAPDH served as a loading control. (D) Western blot showed the overexpression of T7 tagged PCBP1 and the expression level of cellular STAT3α. GAPDH served as a loading control. (E) RT-PCR analysis showed that overexpression of PCBP1 downregulates the expression of STAT3 targets (Bcl-xL and survivin) in MCF-7 cells.