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. 2019 May 7;15(6):1177–1186. doi: 10.7150/ijbs.33103

Figure 5.

Figure 5

STAT3β inhibits the proliferation of OSCC cells and the expression of Bcl-xL and survivin. (A) CAL 27 or SCC-9 cells were stably transfected with STAT3β or control lentivirus. Cells were seeded into 12 well plates on Day 0. Cell numbers were counted on Day 2 and Day 4. (B) The histograms show statistically significant difference between the STAT3β overexpression and control groups on Day 4. Data are the means ± SE, n =3. (C) Western blot showed the overexpression of STAT3β. β-actin served as a loading control. (D) RT-PCR analysis showed that overexpression of STAT3β downregulates the expression of STAT3 targets (Bcl-xl and survivin) in both CAL 27 and SCC-9 cells. (E, F) Effect of STAT3β overexpression on the clonogenic ability of SCC-9 cells. One thousand cells were seeded in 6 cm dishes and cultured for 10 days. Representative images are shown (E). (F) The histograms summarized the numbers of colonies. Data are the means ± SE, n =3. (G) Overexpression of STAT3β decreased cell proliferation induced by PCBP1 knockdown in SCC-9 cells. SCC-9 cells were transfected with STAT3β, empty control plasmid (Vector), PCBP1 specific siRNA and/or non-specific (NS) siRNA. Transfected cells were divided to four groups: STAT3β + siPCBP1, STAT3β + NS, Vector + siPCBP1, and Vector + NS. Cell number was counted at Day 2 and Day 4. (H) The histograms summarized the numbers of cells counted on Day 4. Data are the means ± SE, n =3. (I) Western blot displayed the overexpression of STAT3β and knockdown efficiency of PCBP1. GAPDH served as a loading control.