Table 2.
Image acquisition parameters with the confocal laser spinning disk fluorescence microscope FLUMIAS on the TEXUS-54 suborbital rocket flight mission.
| Parameter | Value | Comment |
|---|---|---|
| Excitation wavelength [nm] | 405/488/561/642 | Cell organelle/structure visualized: nucleus/cytoplasm/lysosomes/F-actin |
| Exposure time [ms] | 15/15/50/50 | - |
| Laser intensity [%] | 100/100/20/100 | - |
| X/Y-step size [µm] | 10 | - |
| Z-step size [µm] | 1 | - |
| 1. Z-stack: Z-stack height [µm] | 90 | Reference run overview stack/in-flight overview stack |
| 1. Z-stack: Image-to-image distance [µm] | 1 | - |
| Image-# 1. Z-stack | 91 | - |
| Z-stack height [µm] | 25.28 | - |
| Image-to-image distance height [µm] | 0.32 | - |
| Image number per Z-stack | 80 | - |
| Number of Z-Stacks per acquisition | 1 | In total four acquisitions (t1–t4) with four wavelengths in-flight |
| Image-# per loop | 320 | four wavelengths each 80 images |
| Image-# per run | 1280 | four loops (t1–t4), each loop with four wavelengths each with 80 images |
| Acquisition time [s] | 21.58 | - |
| Working mode (nominal) | Z-Stack | - |