Figure 3.

Schematic illustration of the engineering method used to transiently transfect MSCs. MSCs were transfected via a neon electroporation system with different miRNA mimics. EVs were collected after 24 h and supernatant, previously centrifuged at 2000 g to eliminate cell debris, was concentrated by 3 kDa filter tube. EVs were then used for in vitro and in vivo experiments or precipitated by Exoquick and used for RNA extraction.