Table 4.
Unique miRNA mature sequences present on the floral transcriptome of sexual and apomictic P. notatum
| miRNA mature sequence | nt | Accession* | Pn | Os | Sb | Si | Family |
|---|---|---|---|---|---|---|---|
| GCUCACUUCUCUUCCUGUCAGC | 22 | MIMAT0015124 | 0 | 0 | 0 | 1 | miR156 |
| GCUCACUUCUCUCUCUGUCAGU | 22 | MIMAT0015129 | 0 | 0 | 1 | 0 | miR156 |
| GCUCACUGCUCUGUCUGUCAUC | 22 | MIMAT0015128 | 0 | 1 | 1 | 1 | miR156 |
| UUGACAGAAGAGAGUGAGCAC | 21 | MIMAT0026186 | 0 | 1 | 0 | 0 | miR156 |
| UUUGGAUUGAAGGGAGCUCUG | 21 | MIMAT0001023 | 0 | 1 | 1 | 1 | miR159 |
| UGCCUGGCUCCCUGUAUGCCA | 21 | MIMAT0000178 | 1 | 0 | 2 | 2 | miR160 |
| UGGAGAAGCAGGGCACGUGCA | 21 | MIMAT0000185 | 0 | 1 | 1 | 1 | miR164 |
| UGGAGAAGCAGGGCACGUGCU | 21 | MIMAT0001034 | 0 | 1 | 1 | 1 | miR164 |
| CAUGUGCCCAUCUUCUCCACC | 21 | MIMAT0015138 | 0 | 1 | 0 | 0 | miR164 |
| CACGUGCUCCCCUUCUCCACC | 21 | MIMAT0015319 | 0 | 1 | 0 | 0 | miR164 |
| UCGGACCAGGCUUCAUUCCUC | 21 | MATAT0001072 | 0 | 1 | 1 | 1 | miR166 |
| UCGGACCAGGCUUCAUUCCCC | 21 | MIMAT0000189 | 0 | 5 | 5 | 5 | miR166 |
| UCGGACCAGGCUUCAAUCCCU | 21 | MIMAT0001037 | 0 | 1 | 2 | 2 | miR166 |
| UGAAGCUGCCAGCAUGAUCUGA | 22 | MIMAT0014072 | 1 | 1 | 1 | 0 | miR167 |
| UGAAGCUGCCAGCAUGAUCUA | 21 | MIMAT0000196 | 0 | 1 | 1 | 2 | miR167 |
| UCGCUUGGUGCAGAUCGGGACC | 22 | MIMAT0001045 | 0 | 1 | 1 | 1 | miR168 |
| UGGGCGGUCACCUUGGCUAGC | 21 | MIMAT0026431 | 0 | 0 | 1 | 1 | miR169 |
| UUGAGCCGCGUCAAUAUCUCC | 21 | MIMAT0014082 | 0 | 1 | 1 | 1 | miR171 |
| GGAUAUUGGUGCGGUUCAAUC | 21 | MIMAT0022877 | 0 | 1 | 0 | 0 | miR171 |
| GUGGUAUUGUUCCGGCUCAUG | 21 | MIMAT0037190 | 0 | 1 | 1 | 1 | miR171 |
| UUGGACUGAAGGGUGCUCCCU | 21 | MIMAT0020761 | 0 | 1 | 1 | 1 | miR319 |
| AAGCUCAGGAGGGAUAGCGCC | 21 | MIMAT0000931 | 0 | 1 | 0 | 0 | miR390 |
| UCCAAAGGGAUCGCAUUGAUC | 21 | MIMAT0000957 | 1 | 1 | 1 | 0 | miR393 |
| GUUCUCCUCAAGCACUUCACA | 21 | MIMAT0015347 | 0 | 1 | 0 | 1 | miR395 |
| UCCACAGGCUUUCUUGAACUG | 21 | MIMAT0001601 | 0 | 0 | 0 | 1 | miR396 |
| UUCCACAGCUUUCUUGAACUG | 21 | MIMAT0000944 | 2 | 1 | 1 | 1 | miR396 |
| UUCCACAGCUUUCUUGAACUU | 21 | MIMAT0000945 | 0 | 1 | 0 | 1 | miR396 |
| UUGAGUGCAGCGUUGAUGAGC | 21 | MIMAT0025996 | 0 | 0 | 1 | 1 | miR397 |
| UGUGUUCUCAGGUCGCCCCCG | 21 | MIMAT0014020 | 0 | 0 | 1 | 0 | miR398 |
| UGCCAAAGGAGAGUUGCCCUG | 21 | MIMAT0000952 | 0 | 0 | 1 | 1 | miR399 |
| UGCCAAAGGAGAGCUGCCCUG | 21 | MIMAT0000992 | 0 | 1 | 1 | 1 | miR399 |
| UGGAAGGGGCAUGCAGAGGAG | 21 | MIMAT0014028 | 0 | 1 | 1 | 1 | miR528 |
| AGAAGAGAGAGAGUACAGCCU | 21 | MIMAT0014030 | 0 | 1 | 1 | 1 | miR529 |
| UGGGUGUCAUCUCUCCUGGAGC | 22 | MIMAT0015366 | 0 | 0 | 1 | 0 | miR1432 |
| UUCCCGAUGCCUCCCAUUCCUA | 22 | MIMAT0011741 | 0 | 0 | 1 | 0 | miR2118 |
| UUCCUGAUGCCUCCCAUUCCUA | 22 | MIMAT0011745 | 0 | 0 | 0 | 1 | miR2118 |
| UUUGGUUUCCUCCAAUGUCUCA | 22 | MIMAT0035533 | 0 | 0 | 0 | 1 | miR2275 |
| CUUGUUUUCCUCCAAUGUCUCA | 22 | MIMAT0037125 | 0 | 1 | 0 | 1 | miR2275 |
| CUUGGUUCCCUCCAAUAUCUCA | 22 | MIMAT0011758 | 1 | 0 | 0 | 0 | miR2275 |
| AGGAUUAGAGGGAACUGAACC | 21 | MIMAT0011769 | 0 | 1 | 1 | 1 | miR2275 |
| CUCUCGCCGGCGUGCGCACUCC | 22 | no hit | 0 | 0 | 1 | 0 | Pn_miR1 |
| AGUGCGCCGCCGUCGAUCUGC | 21 | no hit | 1 | 0 | 0 | 0 | Pn_miR2 |
| UUGACAGAAGAGAGCGAGCAC | 21 | no hit | 0 | 0 | 1 | 1 | Pn_miR3 |
| UGACAGAAGAGAGUGAGCAC | 20 | no hit | 1 | 0 | 2 | 1 | Pn_miR4 |
| UGACAGAAGAGAGAGAGCAC | 20 | no hit | 0 | 0 | 1 | 1 | Pn_miR5 |
| GGGCAAAUCAUCUGGGCUACC | 21 | no hit | 0 | 0 | 0 | 1 | Pn_miR6 |
| UGAGCCGAGCCAAUAUCACUUC | 22 | no hit | 0 | 1 | 1 | 1 | Pn_miR7 |
| UAUUGUCUCGGCUCACUCAGA | 21 | no hit | 0 | 1 | 2 | 2 | Pn_miR8 |
Sequences were detected by using four references: Pn: Paspalum transcriptome; Os: Oryza sativa Japonica genome; Sb: Sorghum bicolor genome; Si: Setaria italica genome
*Accession number was obtained by performing BLASTn analysis (SSEARCH option) of mature sequences on miRBase [48]