. Author manuscript; available in PMC: 2020 Jan 1.

Published in final edited form as: Methods Mol Biol. 2019;1983:133–149. doi: 10.1007/978-1-4939-9434-2_8

Table 1. Example of assay mixtures (per well volume).

Sample	Assay buffer (μL)	SIRT1 (3×) (μL)	Amount of sample (3×) (μL)	NAD⁺/substrate (3×) (μL)

No enzyme	20	0	0	10
Control	10	10	0	10
Resveratrol	0	10	10	10
Suramin	0	10	10	10
Test compound	0	10	10	10