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Knockout phenotypes of secondary metabolite biosynthesis genes induced at later stages of perithecium development. (A) Colony morphologies of deletion mutants (ΔPKS35, ΔPKS7, and ΔTC2) grown on potato dextrose agar [views of culture from above (left); from below (right)]. (B) Perithecium production on carrot agar (left). Asci released from a squashed perithecium (right). Note that ΔPKS7 produced protoperithecia but mature asci failed to develop. Bar = 100 μm.
