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Raman spectrum of bulk 4MBA (top) and SERS spectrum of 4MBA conjugated to AuNPs and dried onto a glass slide (bottom). The peaks of the pH-dependent bands in the SERS spectrum are highlighted: β(COO−) and ν(COO−) in blue and ν(C = O) in red. The spectral region of the ν(SH) band is reported in the insets. The disappearance of the corresponding peak in the SERS spectrum witnesses a successful functionalization of the AuNPs by S-Au covalent binding.
