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. 2019 May 9;9(10):2967–2983. doi: 10.7150/thno.30562

Figure 8.

Figure 8

Lgr5 induces cell proliferation and inhibits Dox-induced apoptosis in HCC cells via interaction with PDCD5. A. SK-Hep1 cells were transfected with Lgr5. Flow cytometric analysis was used to detect apoptosis in cells treated with Dox (0 µg/mL or 1 µg/mL). B. HepG2 cells transfected with shRNA-Lgr5 #1, shRNA-Lgr5 #2 or shRNA-control were treated with Dox (0 µg/mL or 1 µg/mL), and their apoptosis was detected by flow cytometric analysis. C. SK-Hep1 cells transfected with Lgr5 were treated with Dox (1 µg/mL or 0 µg/mL) for 24 h. The cell lysates were analyzed by western blotting with antibodies against apoptosis-related proteins (the ratio of molecule/GAPDH was indicated below). D. HepG2 cells transfected with shRNA-Lgr5 #1, shRNA-Lgr5 #2 or shRNA-control were treated with Dox (1 µg/mL or 0 µg/mL) for 24 h. The western blot shows the expression of apoptosis-related proteins in the cells previously described. *p<0.05, **p<0.01, **p<0.001, t-test.