Figure 1.

Cy5M₂ labels sporozoites in vitro.(A) Fluorescence-microscopy analysis of In vitro labelled Pb sporozoites (Bergreen) expressing GFP (green; cytoplasmic) and a single spot Cy5M₂ (red). Parasite nuclei are stained with Hoechst (blue). Dotted line demarcates the salivary gland edge, separating expelled sporozoites from gland sporozoites. (B) Quantification of fluorescence by Flow Cytometry. Gray lines represent background signal in unlabelled sporozoites. Blue lines show signal after labelling with Hoechst and Cy5M₂. All sporozoites are GFP⁺. Fluorescence intensity on x-axis, Events normalized using FlowJo algorithms in order to account for the differences in numbers of sporozoites measured per sample (% of max; y-axis).