Figure 5.

Labelled sporozoites can be tracked in a human skin explant model. (A) Non-GMO Pf sporozoites, in vitro labelled with Cy5M₂ are injected into human skin explants. Sliced punch biopsies are imaged using confocal microscopy. 2D video microscopy images are analysed using SMOOT_{human skin}. (B) Individual sporozoites tracks visualized by migration pattern (linear in red, sharp turn in yellow slight turn in blue) and quantification of patterns (C). Velocity is tracked (D) and quantified (E) over the full duration of the track. ****: p=<0.0001 using one way analysis of variance (ANOVA) test.