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. 2019 Jun 7;9:199. doi: 10.3389/fcimb.2019.00199

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Copyright © 2019 Guo, Zhang, Eltahan and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CpTEII gene is expressed in all life stages of parasites being tested. (A) The levels of CpTEII transcripts were tested by qRT-PCR in oocysts, sporozoites, and intracellular parasites infecting host cells for various times. The relative levels were normalized to the C. parvum 18S rRNA (Cp18S) and showed in relative to the level in oocysts using the empirical $2^{{- Δ Δ C}_{T}}$ equation. (B) Western blot analysis of purified CpTEII, MBP and crude extracts from C. parvum oocysts and free sporozoites (Spz) using affinity-purified rabbit anti-CpTEII polyclonal antibodies.