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. 2019 Jun 7;9:199. doi: 10.3389/fcimb.2019.00199

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Copyright © 2019 Guo, Zhang, Eltahan and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Indirect immunofluorescence microscopic detection of CpTEII and CpPKS protein in different life stages of C. parvum. Anti-CpTEII antibodies stain highly on some later stage parasites (48 and 72 h post-infection), which are probably macrogamonts with large or unstainable nuclei by DAPI (arrowhead), and relative weak signal can be observed on sporozoites and other parasites. CpPKS staining signal is very faint on sporozoites and weak in 6 and 12 h postinfection stage parasites, and become stronger on some 24, 36, 48, and 72 h postinfection stage parasites (arrow). DIC, differential interference microscopy; DAPI, 4′,6-diamidino-2-phenylindole for staining nuclei; Merged, superimposed images of CpTEII and CpPKS. Bar = 5 μm.