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. 2019 Jun 7;10:1247. doi: 10.3389/fimmu.2019.01247

Figure 3.

Figure 3

Expression of VSIG4, MARCO and CD49a on freshly isolated PBMCs and IHLs (A) (left) Representative histogram of VSIG4 expression on gated CD49a− PBMCs (red), CD49a− ihMφ (light blue), and CD49a+ ihMφ (dark blue). (right) Scatter plot summarizing VSIG4 MdFI on CD49a− PBMCs (red), CD49a− ihMφ (light blue) and CD49a+ ihMφ (dark blue) (n = 5). (B) (left) Representative histogram of MARCO expression on gated CD49a− PBMCs (red), CD49a− ihMφ (light blue) and CD49a+ ihMφ (dark blue). (right) Scatter plot summarizing MARCO MdFI on CD49a− PBMCs (red), CD49a− ihMφ (light blue) and CD49a+ ihMφ (dark blue) (n = 6). (C) (left) Representative histogram of CD49a expression on gated VSIG4− PBMCs (red), VSIG4− ihMφ (light blue) and VSIG4+ ihMφ (dark blue). (right) Scatter plot summarizing CD49a MdFI on VSIG4− PBMCs (red), VSIG4− ihMφ (light blue) and VSIG4+ ihMφ (dark blue) (n = 6). (D) (left) Representative histogram of MARCO expression on gated VSIG4− PBMCs (red), VSIG4− ihMφ (light blue) and VSIG4+ ihMφ (dark blue). (right) Scatter plot summarizing MARCO MdFI on VSIG4− PBMCs (red), VSIG4− ihMφ (light blue) and VSIG4+ ihMφ (dark blue) (n = 6). (E) (left) Representative histogram of CD49a expression on gated MARCO− PBMCs (red), MARCO− ihMφ (light blue) and MARCO+ ihMφ (dark blue). (right) Scatter plot summarizing CD49a MdFI on MARCO− PBMCs (red), MARCO− ihMφ (light blue) and MARCO+ ihMφ (dark blue) (n = 6 for PBMCs and MARCO− IHLs, n = 5 for MARCO+ IHLs). (F) (left) Representative histogram of VSIG4 expression on gated MARCO− PBMCs (red), MARCO− ihMφ (light blue) and MARCO+ ihMφ (dark blue). (right) Scatter plot summarizing VSIG4 MdFI on MARCO− PBMCs (red), MARCO− ihMφ (light blue) and MARCO+ ihMφ (dark blue) (n = 5 for PBMCs and MARCO− IHLs, n = 4 for MARCO+ IHLs). One sample was excluded due to the low proportion of MARCO+ cells. Median is depicted and error bars indicate the min-max range, p-value determined by Mann-Whitney test (PBMCs vs. IHLs) and Wilcoxon matched-pairs signed rank test when comparing IHL populations.