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. Author manuscript; available in PMC: 2019 Jun 14.
Published in final edited form as: J Alzheimers Dis. 2019;67(3):1021–1034. doi: 10.3233/JAD-180820

Figure 2. 24-hour exposure to the activated BV2 cell conditioned medium did not alter SH- SY5Y cell AβPP levels but reduced sAβPPα.

Figure 2.

(A) AβPP mRNA levels from SH-SY5Y cells maintained for 24 hours in conditioned media from activated and unactivated BV2 cells were comparable. n=6 for both groups. (B) Representative Western blot for ApPP revealed a double band pattern. (C) Densitometry analysis for both the upper and lower ApPP Western blot bands revealed equivalent AβPP protein levels in SH-SY5Y cells maintained in conditioned media from activated and unactivated BV2 cells. n=6 for both groups. (E) Western blot for sAβPPα. (F) After normalizing to the Amido Black density from each lane, densitometry analysis of the sAβPPα Western blot bands revealed sAβPPα levels were lower in the medium from SH-SY5Y cells maintained in activated BV2 cell-conditioned medium than they were in the medium from SH-SY5Y cells maintained in unactivated BV2 cell-conditioned medium. n=6 for both groups. *p<0.05. Ctrl=control, CM=conditioned medium. Error bars are SEM.