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. Author manuscript; available in PMC: 2019 Jun 14.
Published in final edited form as: Nat Methods. 2019 Apr 29;16(5):369–379. doi: 10.1038/s41592-019-0395-x

Figure 6. Cryo-FIB milling of a thick crystal.

Figure 6.

(A) Sample preparation for MicroED using a cryo-FIB to mill down thick crystals to a few hundred nanometers (left and right). (B) Following cryo-FIB milling, the grid would be loaded into the TEM and diffraction data would be collected from the thin lamella. (C-D) Final structure of proteinase K.