| Component | Volume (μl) | Final concentration |
|---|---|---|
| H2O | 12.6 | |
| 5x Herculase Buffer | 4 | 1 x |
| dNTPs | 0.2 | 1 mM |
| P5 | 0.5 | 1 μM |
| P7.2 | 0.5 | 1 μM |
| Herculase II | 0.2 | |
| Template (pooled, column purified PCR2 product from Step 50) | 200 ng | |
| Total | 20 | |
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