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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Exp Gerontol. 2019 Feb 8;120:101–106. doi: 10.1016/j.exger.2019.02.001

Figure 1. Potassium restriction evokes vacuolar acidification and confers resistance to V-ATPase inhibition.

Figure 1.

In each panel, the y-axis reports the proportion of yeast cells stained by the vacuolar dye quinacrine with the intensity (in arbitrary units) shown on the x. Blank, no quinacrine. (A) Wild-type yeast cultured in standard medium (control) or potassium restricted medium (low-K). (B) Data are as in (A) except that after growth in low-K or control medium, cells were subject to acute treatment with concanamycin A (ConcA) or left untreated. (C) Wild-type (WT) or isogenic potassium transporter mutants (trk1, trk2) cultured in standard potassium-replete medium. **, p < 0.001; *, p < 0.02 in a comparison of the indicated sample to cells cultured in control medium (A), cells cultured in control medium and then treated with concanamycin A (B), or wild-type cells (C).