Fig. 2.

A κB-transgene is activated during cerebellum development. Total RNAs (30 μg) from adult brain, 5 and 10 PN cerebella from mice carrying a 3xκB-human β-globin (B.glob) transgene, were used in RNase protection experiments with a human β-globin probe. Transgene expression is not detected in the adult brain (lane 2), is slightly detectable in 5 PN cerebellum (lane 3), and is increased in 10 PN cerebellum (lane 4). Lane 1, RNA extracted from the S194 mouse plasmacytoma cell line transiently transfected with the 3xκB β-globin plasmid (positive control).