Fig. 4.

C3 transferase action in DRG neurons. DRG neurons were triturated, cultured, and assayed as described in Figure 2. The data are averages ± SEM for two to four separate experiments.A, Growth cone collapse after the indicated concentrations of C3 transferase were present during the trituration of DRG neurons was determined in the presence and absence of 200 pm collapsin-His₆ (Col).B, Growth cone collapse was determined after the trituration of DRG neurons with buffer, 4 μg/ml C3 transferase, 5 mg/ml V14rho, or both proteins and after a subsequent exposure of the neurons to 0 (gray bars) or 200 pm(solid bars) collapsin-His₆. Results for trituration with both C3 transferase and either V12rac or V12cdc42 are also given. C, Average total neurite outgrowth per cell for neurons triturated as described in B was determined after plating with (gray bars) or without (solid bars) 200 pmcollapsin-His₆.