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. 2019 Jun 14;14(6):e0214173. doi: 10.1371/journal.pone.0214173

Fig 6. Immunolabeling and quantification of TRAP+OC in the control and ZA treated mice.

Fig 6

Senescent 129 Sv-WT female mice received IP injections of 0.9% saline solution (Vehicle) or 250 μg/Kg one a week and upper right incisor were removed after 4 weeks of Vehicle or ZA treatments. Mice were euthanized for maxillary bones removal after 7days and 21days post tooth extraction. A) Representative transversal sections are observed throughout days 7 and 21, from the central area of alveolar sockets, for TRAP+OC in Control and ZA group. B-E) Quantitative and comparative analysis of detached TRAP+ OC (B), attached TRAP+ OC (C) and the Total of TRAP+OC considering the sum of detached and attached TRAP+ OC (D) in control vs ZA treated mice at days 7 and 21 post-extraction. Results are presented as the means (±SD) of area density (%). Symbol *indicate a statistically significant difference vs control (p<0.05). DAB chromogen and counterstaining with Harris’ Hematoxylin.