Fig 4. Quality of DNA extracted from cultured human cancer cells using different DNA extraction methods after fixation for 90 days.
MCF-7, Capan-1, and FTC133 cells were fixed in CytoRich Red solution at a density of 6 × 103 cells/mL for 3 months at 4°C, and then the quality of extracted DNA was evaluated based on the QC scores and amplifiable DNA concentrations. The Maxwell 16 FFPE Tissue LEV DNA Purification kit (Promega) yielded the highest DNA quality. *, p < 0.05; **, p < 0.01. Qiagen, DNeasy Blood & Tissue kit; Takara, NucleoSpin Tissue XS kit; Beckman, Agencourt FormaPure DNA kit; Promega, Maxwell 16 FFPE Tissue LEV DNA Purification Kit.